Mar. 1, 2007 — Malvern Instruments‘s Zetasizer nano particle and molecular characterisation system now offers a chromatography flow-mode option that lets the system operate as an in-line chromatography detector. The new option allows the coupling of dynamic light scattering with size exclusion chromatography, enabling Absolute Size Exclusion Chromatography (ASEC). This means protein size can be measured directly rather than indirectly through column calibration or additional data from a second detector. It also means that molecular weight standards are no longer required to identify oligomeric species or determine protein purity.
The instrument can be added to any SEC set-up; no column calibration is required and Malvern promises that its software makes the measurement process easy.